'Lab Report on Effect of Enzymes Essay\r'

'ntroduction\r\nThe enzyme catalase is present in cells in commit to speed the breakdown of total heat henry peroxide (H2O2), which is a toxic chemical to the human body. When heat content peroxide is broken down, the end products atomic number 18 Water (H2O) and group O (O2). In this report, the response of catalase to atomic number 1 peroxide is beingness leavened. Furthermore, the effects of temperature, concentration, and pH level on this reaction are being considered. We hypothesize that he experiments allow show that Catalase speeds the breakdown of hydrogen peroxide to piddle and oxygen, and the oxygen will produce a larger bubbling effect in the solutions where the optimum conditions for enzymatic activity are present. These optimum conditions should be in warm temperature, high concentration, and high pH levels. First, the reaction of catalase to hydrogen peroxide is raveled with no superfluous factors. Then, to field of operation the effect of temperature, triplet ranges of temperature are introduced in the second experiment. Next, to try out the effect of concentration, triad incompatible levels of catalase are studied in the third experiment. And finally, to study the effect of pH, solutions with three levels of hydrogen concentration are try oned in the fourth experiment, once more with hydrogen peroxide and catalase.\r\nMethods\r\nExperiment 1: To demonstrate the catalase-hydrogen peroxide reaction with no additional factors, we marked three test tubings to the 1 cm and 5 cm marks. Catalase was added to the 1 cm mark of test pipework 1 and test electron subway system 3, plot of ground test tube-shaped structure 2 was fill to the 1 cm mark with wet. Hydrogen peroxide was added to the 5 cm mark in test tube 1 and test tube 2, while a saccharose solution was used to fill test tube 3. All test tubes were mixed and we waited 20 seconds for bubbling. ladder tube 1 represented the experiment, while test tube 2 was the nega tive bear for hydrogen peroxide, and test tube 3 was the negative control for the catalase. Experiment 2: To test the effect of temperature on enzyme activity, the solution created in test tube 1 of the previous experiment was recreated in three split up test tubes. judge tube 1 was set in an ice bath. Test 2 was situated in warm water. Test tube 3 was placed in boiling water. Experiment 3: To test the effect of concentration, three separate test tubes were set of to have three different concentrations of the reactant (catalase), while the substrate was consistent across all three test tubes.\r\nTest tube 1 was filled in the same fashion as test tube 1 in the first experiment. Test tube 2 was then filled to 2 cm with catalase and then to 7 cm with hydrogen peroxide. Test tube 3 was filled to 3 cm with catalase, and then to 8 cm with hydrogen peroxide. Experiment 4: Finally, to test the effect of pH Level on enzyme activity, three test tubes were again created. The concentration of catalase and hydrogen peroxide are again the same as in test tube one in the first experiment. However, 2 cm of water adjusted to different pH levels were added. In test tube 1, 2 cm of water with pH of 3 is added to the 1 cm of catalase and 4 cm of hydrogen peroxide. In test tube 2, 2 cm of water adjusted to the pH adjusted to 7 was added to 1 cm of catalase and 4 cm of hydrogen peroxide. In test tube 3, 2 cm of water adjusted to the pH adjusted to 11 was added to 1 cm of catalase and 4 cm of hydrogen peroxide.\r\nDiscussion\r\nThe discussion separate takes your interpretation of the results and provides the answer to the research question draw in the introduction. Specifically, discuss whether or not your hypotheses were supported. Also, include a comparison to previous studies, discuss the limitations of your study (briefly), and detail unexpected findings. Finally, summarize your conclusions and discuss the import of your results in a broader context. Use the appropriate reach as described above.\r\nReferences\r\nThe references section is a keep down of all references cited in the text. Arrange references alphabetically consort to author name, not chronologically. The name of the journals containing the cited papers should be written out in full. Town/metropolis and country names should be provided for non-journal references. Each clause reference should be given as in the following example:\r\nAlfano J.R., Collmer A. (2004) Type III secretion system effector proteins: double agents in bacterial disease and plant defence. Annual Review Phytopathology, 42, 385â€414.\r\nBooks or other non-serial publications which are quoted in the references must be cited as follows:\r\nGage J.D., Tyler P.A. (1991) Deep-sea Biology: A Natural History of Organisms at the Deep-sea Floor. Cambridge University Press, Cambridge, UK: 504 pp.\r\nLester R.N., Hasan S.M.Z. (1991) filiation and domestication of the brinjal eggplant, Solanum melongena, from S. incanum, in Africa and Asia. In: Hawkes J.G., Lester R.N., Nee M., Estrada N. (Eds), Solanaceae III: Taxonomy, Chemistry, Evolution. Royal botanic Gardens, Kew; London, UK: 369â€387.\r\n'